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zetaview® basic nta device  (Particle Metrix)

 
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    Particle Metrix zetaview® basic nta device
    Zetaview® Basic Nta Device, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zetaview%C2%AE+basic+nta/zetaview+nta+instrument/pmc12116862-51-20-25
    Average 90 stars, based on 1 article reviews
    zetaview® basic nta device - by Bioz Stars, 2026-08
    90/100 stars

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    Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView <t>Nanoparticle</t> Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.
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    Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView <t>Nanoparticle</t> Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.
    Zetaview Basic Nta, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView <t>Nanoparticle</t> Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.
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    Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView <t>Nanoparticle</t> Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.
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    Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView <t>Nanoparticle</t> Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.
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    Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView Nanoparticle Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.

    Journal: Microbiology Spectrum

    Article Title: Antigen 43 associated with Escherichia coli membrane vesicles contributes to bacterial cell association and biofilm formation

    doi: 10.1128/spectrum.01890-24

    Figure Lengend Snippet: Characterization of E. coli Ag43 −ve and Ag43 +ve MVs. MVs isolated from ( A ) E. coli HEHA16 pBAD/MycHisA (Ag43 −ve ) and ( B ) E. coli HEHA16 pBAD/MycHisA:: agn43 (Ag43 +ve ) were visualized by transmission electron microscopy. Scale bar = 100 nm. Images are representative of three biological replicates. ( C ) The number of Ag43 −ve and Ag43 +ve MVs produced per 10 10 bacterial cells was determined by ZetaView Nanoparticle Tracking Analysis (NTA). Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test. ( D ) The number of viable Ag43 −ve and Ag43 +ve E. coli HEHA16 bacteria present in individual cultures from which MVs were isolated was determined by viable counts. Data show CFU/mL of individual cultures, and the mean ± SEM of three biological replicates. ns, not significant. ( E ) The size distribution of Ag43 −ve MVs and Ag43 +ve MVs was determined using ZetaView NTA. Data represent the mean of three biological replicates. ( F ) Quantification of the size distribution of Ag43 −ve MVs (squares; open bars) or Ag43 +ve MVs (circles; grey bars) by ZetaView NTA. Data are represented as the mean ± SEM of three biological replicates. * P < 0.05, unpaired t -test within each size range.

    Article Snippet: MV size and concentration were determined using the nanoparticle tracking analyzer ZetaView basic PMX-120 NTA (Particle Metrix, Germany), as previously ( ).

    Techniques: Isolation, Transmission Assay, Electron Microscopy, Produced, Bacteria